The effects of tarantula cubensis extract on renal ischemia reperfusion injury in rats

Background/aim: Renal ischemia-reperfusion (IR) related acute kidney injury (AKI) is an important health problem and has not yet been fully treated. Tarantula cubensis extract (TCE) a homeopathic drug that antiinflammatory antioxidant effects. This study aimed to investigate the effects of TCE on renal in rats. Materials methods: was carried out 48 Spraque-Dawley male rats, which were divided into six groups. The first, second, third groups control, sham, IR groups, respectively. Group four received 0.2 mL 96% ethanol. five ischemia reperfusion 0.01 0.1 mg per rat (which correspond approximately 0.04 mg/kg, 0.4 mg/kg), Tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1ß), total status (TAS), oxidant (TOS) levels tissue measured by enzyme-linked immunosorbent assay (ELISA). Oxidative stress index (OSI) obtained proportioning TAS TOS. Superoxide dismutase (SOD), myeloperoxidase (MPO) activities, malondialdehyde (MDA) determined manual spectrophotometric methods. histopathological changes evaluated via hematoxylineosin immunohistochemical staining. Results: In group, TNF-α IL-1ß significantly higher than control group (p < 0.0001 for both), low both) high dose administration decreased these markers. Low doses OSI values compared with = p 0.001 respectively). Although MDA levels, it statistically significant. MPO decreased. addition, found prevent hemorrhage, cast formation, dilatation caused tissues stained hematoxylin-eosin. And also, most intense nuclear kappa B (NFKB) caspase-3 immunopositivity both Conclusion: showed protective effect inhibiting inflammation against damage tissues, there no clear oxidative stress. Larger more detailed studies are needed clarify issue.